Magnetic resonance and bioluminescence imaging of macrophage homing to experimental abdominal aortic aneurysms
نویسندگان
چکیده
Methods Murine AAAs were created via intra-aortic infusion of porcine pancreatic elastase and aortic size was monitored by ultrasound on post-op day (POD) 3, 7, and 14. Mice were imaged after injection of prepared peritoneal macrophages. For BLI, macrophages were from transgenic mice expressing luciferase, with injection on POD 1 and imaging at POD 3, 7, and 14 by in situ BLI (AAA N=17, sham control N=15). For MRI, macrophages were labeled with iron oxide particles (Feridex), with injection on POD 13 and imaging at POD 14 by in vivo 7 T MRI (AAA N=8, sham N=5). Mice were sacrificed after imaging for histological analysis.
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